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. 2018 Dec 20;12(3):453–461. doi: 10.1016/j.tranon.2018.12.002

Figure 6.

Figure 6

Immunohistochemical analysis of PC-3 xenograft tumor tissues. (A) Representative microscope images of TUNEL-stained tumor tissue sections derived from mice treated with PBS, IL-24, or IL-24-iRGD. TUNEL-positive nuclei are stained brown, and TUNEL-negative nuclei are stained blue (n = 6; magnification, ×400). (B) Quantitative analysis of TUNEL staining in each group. The percentage of TUNEL-positive cells was counted from 100 tumor cells selected at random in each tissue section, and five sections were counted per tumor (n = 6). (C) Expression of cleaved caspase-3 in PC-3 tumor xenograft tissues following treatment with PBS, IL-24, or IL-24-iRGD. Brown staining indicates positive cleaved caspase-3 expression, and blue staining indicates cell nuclei. Representative images from each group are shown (n = 6; magnification, ×400). (D) Quantitative analysis of cleaved caspase-3 staining in each treatment group. A total of five fields of view for each tumor tissue section were selected at random. IOD values of positively stained regions were calculated using Image-Pro Plus software. Data are expressed as the mean ± SD. Scale bar = 50 μm, n = 6. ⁎⁎⁎P < .001, as indicated.