Table 1.

Iron parameters of Tfr2^BMKO/Hbb^th3/+ mice 9 and 22 weeks after BMT

		TS, %	LIC, μg/g	SIC, μg/g	KIC, μg/g	HIC, μg/g	Hamp −ΔCt	Hamp/LIC
9 wk after BMT	Hbbth3/+	49.9 ± 4.5	529.2 ± 23.7	4039 ± 69	248.0 ± 29.3	229.2 ± 10.8	5.79 ± 0.12	1.01 ± 0.09
	Tfr2BMKO /Hbbth3/+	41.1 ± 2.3	530.8 ± 72.4	3981 ± 172	249.8 ± 23.8	215.7 ± 15.1	5.90 ± 0.24	1.18 ± 0.14
22 wk after BMT	Hbbth3/+	31.1 ± 1.7	961.3 ± 98.2	7234 ± 779	401.3 ± 38.1	388.7 ± 19.4	5.03 ± 0.29	0.50 ± 0.05
	Tfr2BMKO /Hbbth3/+	30.3 ± 5.3	658.6 ± 83.0*	2383 ± 647***	263.7 ± 21.4*	382.9 ± 29.7	2.80 ± 0.96*	0.31 ± 0.19

Mice were analyzed 9 and 22 weeks after transplantation with thalassemic (Hbb^th3/+) or Tfr2^−/−/Hbb^th3/+ (Tfr2^BMKO/Hbb^th3/+) BM. Mice were fed a standard diet. The table reports: TS, LIC, SIC, KIC, HIC, and mRNA expression of hepcidin (Hamp) relative to Hprt1 and relative to LIC (Hamp/LIC). Mean values plus or minus SEs are indicated.

HIC, heart iron content; Hprt1, hypoxanthine phosphoribosyltransferase 1; KIC, kidney iron content; mRNA, messenger RNA.

Asterisks refer to statistically significant differences between age-matched Tfr2^BMKO/Hbb^th3/+ and Hbb^th3/+ mice: *P < .05; ***P < .005.