. 2018 Dec 20;18:396. doi: 10.1186/s12888-018-1974-6

Table 1.

PCR primers, PCR conditions and sequencing primers for genotyping five gene using pyrosequencing

	Primer for PCR		Sequencing primer for Pyrosequencing	Annealing temperature
DRD4 VNTR	Forward	5’-GCGACTACGTGGTCTACTCG-3′	–	60 °C
DRD4 VNTR	Reverse	5’-AGGACCCTCATGGCCTTG-3′	–	60 °C
DAT1 VNTR	Forward	5’-TGTGGTGTAGGGAACGGCCTGAG-3′	–	65 °C
DAT1 VNTR	Reverse	5’-CTTCCTGGAGGTCACGGCTCAAGG-3’	–	65 °C
NET8 (rs5569)	Forward	5’-B-GTGAAGAGTTTCCGGTGTCGC-3’	5’-GCATGGAGGCTGTCATC-3’	57 °C
NET8 (rs5569)	Reverse	5’-GAGGCATGGAGGCTGTCA-3’	5’-GCATGGAGGCTGTCATC-3’	57 °C
CHRNA4 (rs1044396)	Forward	5’-CAGCCCTCTCCGTGCAAAT-3’	5’-CTCTTCGGTGTCCCC-3’	57 °C
CHRNA4 (rs1044396)	Reverse	5’-B-GGTGCTGCGGGTCTTGAC-3’	5’-CTCTTCGGTGTCCCC-3’	57 °C
CRHR1 (rs28364027)	Forward	5’-B-AGGAACCCTGGAGACAGAAGT-3’	5′-GGTTAAGTGAGGGGAA-3’	55 °C
CRHR1 (rs28364027)	Reverse	5′-TGGTATGGGGTGGTTAAGTGA-3’	5′-GGTTAAGTGAGGGGAA-3’	55 °C

PCR polymerase chain reaction, B biotin