Figure 8.

Fluorescence images of rat embryonic fibroblasts cultivated for 4 days on CNTT–HA (row A, B), CNTT–BGN (row C, D), and CNT–BGN/HA (row E, F) scaffolds. YFP-paxillin transfected REF cells (focal adhesion sites; yellow) were stained with DAPI (nuclei; blue) and phalloidin (actin filament; red). Fluorescence imaging took place in optical sections of 200 μm from the surface inside the material. Small paxillin clusters are visible in each optical focal plane. Imaging of actin reveals spanned cells with actin meshes. To show more details, fluorescence images of two different regions of each scaffold are shown in two rows (scale bars: 10 μm).