Figure 4.
Surface density of cDNA-MB detected from CV experiments using PLL-OEG(24.9) PLL-OEG(28.1)-Mal(3.1), PLL-OEG(29.1)-Mal(5.5), and PLL-OEG(19.4)-Mal(9.1) polymers. Calculations were done by means of eq 2 (Experimental Section). All gold substrates were prepared using 0.3 mg/mL of modified PLL, 1 μM PNA thiol solution (activated by TCEP), and 1 μM MB-modified cDNA solution in PBS at pH 7.2. Fresh solutions of 0.1 M NaClO4 were used as electrolyte. Data points and given single standard deviations are based on 3–4 measurements each.