Fig. (4).

Anti-CD49d injections reduced astrogliosis in APP/PS1 mice. C57BL/6 and APP/PS1 mice were injected intravenously (tail-vein) with Saline, IgG isotype control (purified NA/LE Rat IgG2b) (I.C.) or 2 mg/kg purified NA/LE rat anti-mouse CD49d (anti-CD49d) once a week for 4 weeks. Right brain hemispheres from all the mice were fixed and serially sectioned. Astrogliosis was assessed by immunostaining using anti-GFAP antibody (4A). Representative images from 3-6 animals per group are shown at 20X magnification. Parietal cortex from left brain hemispheres was dissected out, lysed and western blotted using anti-GFAP antibody (4B) with anti-α-tubulin as loading control. Optical densities for each antibody were normalized, averaged and graphed ± SD from 3-6 animal per group (*p<0.05).