Fig. 2.

Restoration of miR-133a-3p expression inhibits proliferation,migration and invasion abilities of GC cell and organoid model. (a). Expression levels of miR-133a-3p among several GC cell lines. (b). Proliferation rate was analyzed by CCK8 assay.miR-133a-3p overexpression suppressed the proliferation of GC cells while the miR-133a-3p-IN had the opposite effect. (c) DNA synthesis was analyzed by Edu assay.miR-133a-3p overexpression suppressed the DNA synthesis of GC cells while the miR-133a-3p-IN had the opposite effect, scale bar = 100 μm. (d). The clonogenicity of both miR-133a-3p mimic and inhibitor transfected cells were assessed. (e). Migration and Invasion abilities of GC cells were supprresed under miR-133a-3p mimics while the miR-133a-3p-IN had the opposite effect, scale bar = 200 μm. (f). We found that overexpression of miR-133a-3p could significantly block the growth of GC organoid, whereas miR-133a-3p knockdown could promote GC growth, scale bar = 50 μm. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001