Fig. 5.

Identification of GABARAPL1 as the potential target of miR-133a-3p which is responsible for the autophagy activation and mediating the proliferation and metastasis abilities of GC cells. (a,b). Clonogenicity assay demonstrated that the positive role on GC clonogenic formation of miR-133a-3p inhibitors was successfully reversed by GABARAPL1 siRNA and HCQ. (c). Edu assay demonstrated that the positive role on GC DNA synthesis of miR-133a-3p inhibitors was successfully reversed by GABARAPL1 siRNA and HCQ, scale bar = 100 μm. (d). Transwell assay proved that the positive role on GC metastasis of miR-133a-3p inhibitors was successfully reversed by GABARAPL1 siRNA and HCQ, scale bar = 200 μm. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001