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. 2019 Jan;25(1):92–101. doi: 10.3201/eid2501.181347

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This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Maximum-likelihood phylogenetic trees showing 2 distinct FeLV-Pco clades in Florida panthers, Florida, USA. A) Full-genome phylogeny indicates Florida FeLV-A sequences are monophyletic. Historic and contemporary FeLV outbreak sequences reside in 1 clade, and a second clade consists solely of contemporary FeLV outbreak sequences. B) Genotyping sequence phylogeny generated from concatenating 3 regions of ≈100 bp (LTR-gag, gag, and env) compares full-genome isolates demonstrated in panel A in addition to 4 individual sequences. Black text indicates FeLV from domestic cats, orange indicates FeLV from panthers during the historic outbreak (2002–2004), and red indicates FeLV from panthers during the contemporary outbreak (2010–2016). Bold indicates isolates sequenced in this study. GenBank accession numbers are provided. FeLV, feline leukemia virus; LTR, long terminal repeat.