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. 2018 Dec 11;25(11):3136–3147.e5. doi: 10.1016/j.celrep.2018.11.067

Figure 7.

Figure 7

Neutralization of EEEV Infection

(A and B) Focus reduction neutralization assay. (A) Bivalent mouse IgG mAbs inhibit infection efficiently unlike (B) monovalent Fabs (hepatitis C virus-specific mAb H77.39, negative control). The neutralization experiments were performed twice, each time in duplicate, and the curves report mean values and SDs.

(C) Steric restrictions on Fab binding to alphaviruses. E1-E2 spike, gray-yellow; Fab, blue cylinder; Fab quasi-2-fold axis, broken line. Tangential binding does not cause significant clashes between domain A Fabs bound to a spike (upper left) unlike radial binding (upper right). In contrast, tangential binding of domain B Fabs may encounter clashes with Fabs bound to neighboring spikes and even with neighboring spikes (lower left), unlike radial binding (lower right).