FIG. 6.

In vitro model of stem cell microenvironment is reinforced by 3D cell culture formation and coculture of hMSCs and Jurkat cells. (A) A 3D hMSC configuration is achieved by fabricating pillar constructs that are 500 μm apart from each other, as shown by the formation of cell “bridges” structure between the pillars in the 3D confocal reconstruction (i) and the corresponding confocal sections in x–y (ii), y–z (iii), and x–z (iv) planes. Dotted lines in (ii) and (iv) indicate edges of pillars. (B) Proliferation of Jurkat cells is enhanced in coculture with hMSCs (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). 3D, three-dimensional.