Table 1.
Plasmid vectors used for fungal transformation
| Plasmid series name | Fungal selection marker | Approximate average vector size | Features | Fungi type | References |
|---|---|---|---|---|---|
| pAG | HIS3, LEU2, TRP1, URA3 | 7 kb | More than 200 options; contains fluorescence reporters | Yeast | Alberti et al. (2007) |
| pXP | HIS3, LEU2, MET15, TRP1, URA3 | 5 kb | Recycling of selection markers by loxP/Cre technology | Yeast | Fang et al. (2011) |
| EasyClone | HIS3, LEU2, LYS5, URA3 | 6 kb | Multiple integrations; recycling of markers. | Yeast | Jensen et al. (2014) |
| EasyClone2.0 | amds, ble, dsd, hph, kan, nat | 6 kb | Compatible with prototrophic strains; recycling of markers | Yeast | Stovicek et al. (2015) |
| EasyCloneMulti | Kl.URA3‐degradation signal | 6 kb | Integrates into Ty sequences; recycling of markers. | Yeast | Maury et al. (2016) |
| pRG | HIS3, LEU2, LYS2, MET15, URA3 | 6 kb | Modular design; multiple integrations; recycling of markers | Yeast | Gnügge et al. (2016) |
| pWEF | hph | 12 kb | Binary vector | Filamentous | Lv et al. (2012) |
| pDESTR | hph | 5 kb | Gene targeting and disruption | Filamentous | Abe et al. (2006) |
| pCBGW‐GFP | hph | 8 kb | Expression vector | Filamentous | Zhu et al. (2009) |
| pGWB2‐GFP | hph | Not shown | Binary vector | Filamentous | Zhu et al. (2009) |
| pEX1 and pEX2 | pyrG | 10 kb | Binary vector | Filamentous | Nguyen et al. (2016) |
| pBI‐hph | hph | 15 kb | Binary vector | Filamentous | Zhong et al. (2007) |
| pALS‐1 | qa‐2+ | 13 kb | Tested in N. crassa. | Filamentous | Sthol and Lambowitz (1983) |