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. 2018 Dec 6;9:2845. doi: 10.3389/fimmu.2018.02845

Figure 2.

Figure 2

Multi-color flow cytometry for the identification and quantification of total circulating monocyte subsets. Monocyte subsets were identified using a 6-color staining strategy in freshly-isolated PMBC from 169 adults with No CKD (n = 27) or CKD 1–5 (n = 142). (A) Representative dot plots are shown to illustrate the gating strategy for the identification and quantification of total monocytes (Upper Panels) and of the three currently-recognized monocyte subsets. (B–D) Graphs are shown of absolute numbers of classical, intermediate, and non-classical monocytes respectively expressed as cells per mL of whole blood for the two groups. Graphs illustrate the median, IQR, and upper and lower limit for each leukocyte subtype for No CKD and CKD groups (box and whisker plots) with data-points for individual subjects superimposed (triangles). Statistical comparisons performed using Mann-Whitney U-test. ns p > 0.05, **p < 0.01.