Figure 5.

Effect of TAT-Ngn2 on the expression of NGF, brain-derived neurotrophic factor (BDNF), the level of phosphorylation of tropomyosin-related kinase B (TrkB) and mitochondrial cytochrome C (CytC) leakage in vivo (n = 6 for each group). (A,C) Western blotting showing NGF and BDNF expression in the hippocampus at 48 h after reperfusion. (B,D) Statistical analysis of NGF and BDNF expression in every group. (E) Western blotting showing the p-TrkB expression in the hippocampus at 48 h after reperfusion. (F) Statistical analysis of p-TrkB expression in every group. (G,I) Western blotting showing the level of CytC in the cytoplasm (C CytC) and CytC in the mitochondria (M CytC) in the hippocampus at 48 h after reperfusion. (H,J) Statistical analysis of C CytC and M CytC levels in every group. Band densities were measured using the ImageJ program and normalized to COX IV or to β-actin (*P < 0.05 vs. sham group, ^#P < 0.05 vs. BCCAO group. The data from the Western blotting studies were analyzed using one-way ANOVA with Dunnett’s test). (K) Electron microscopic image of the ultrastructure of hippocampal neurons at 48 h after reperfusion. Arrowheads indicate swollen mitochondria in the cytoplasm. The mitochondria swelled to a spherical shape. Scale bar = 1 μm. (L) Statistical analysis of the number of swollen mitochondria in every group (n = 6 for each group, serially five sections were observed and analyzed per animal; *P < 0.05 vs. sham group, ^#P < 0.05 vs. BCCAO group). The data were analyzed by one-way analysis of variance followed by Tukey’s multiple comparisons tests.