. 2018 Dec 5;37(12):973–981. doi: 10.1089/dna.2018.4376

Table 3.

Haplotype Effect on TF Binding Activity Expressed as a Ratio of RLU of the Tested Haplotype Over the RLU of Haplotype 1

	Haplotype construct
TFs	Haplotype 1^a	Haplotype 4^b	Haplotype 29^b	Haplotype 30^b
TFIID	5125	0.22	0.63	0.55
TR	4703	0.16	1.04	0.51
NF-1	4686	0.29	1.62	0.87
ER	4499	0.13	0.63	0.52
Stat3	4254	0.36	0.49	0.60
TCF/LEF	4061	0.31	0.36	0.40
Brn-3	3974	0.14	0.62	0.48
C/EBP	3851	0.08	0.20	0.33
Myb	3438	0.18	0.58	0.40
YY1	3345	0.32	1.38	0.53
NF-E2	3233	0.25	1.02	0.35
Pax-5	3216	0.23	2.10	0.48
Oct4	3169	0.26	0.62	0.83
FAST-1	2940	0.20	0.41	0.45
GAS/ISRE	2856	0.66	0.83	0.35
GATA	2855	0.63	1.21	0.39
SATB1	2849	0.45	0.26	0.65
PXR	2733	0.33	1.76	0.67
Sp1	2473	0.22	0.31	1.02
CAR	2408	0.22	1.14	0.42
Pit	2394	0.61	1.04	0.68
Ets	2366	0.18	0.81	0.63
MEF2	2202	0.16	0.44	0.69
Stat1	2083	0.72	1.05	0.20
E2F-1	2076	0.38	0.72	0.42
p53	2028	0.24	1.11	0.54
CDP	1950	1.00	1.11	2.20
Stat6	1898	0.45	0.60	1.07
CREB	1653	0.29	1.32	2.89
IRF	1646	0.51	0.56	0.65
SMAD	1552	0.34	0.69	1.14
CBF	1429	0.50	0.64	0.55
NFAT	1418	0.31	0.75	1.04
ATF2	1380	0.51	0.66	0.72
HNF4	1361	0.39	0.80	0.94
SRF	1335	0.24	2.99	1.17
EGR	1276	0.69	0.74	0.92
HIF	1272	0.56	0.66	0.57
Pbx1	1218	0.84	1.70	0.71
NFkB	1217	0.45	0.61	0.88
Stat5	1178	0.93	1.18	1.25
PPAR	1133	0.51	0.77	0.74
Myc-Max	1091	0.57	1.69	1.01
AP1	1058	1.96	2.05	1.66
Stat4	826	1.32	1.30	1.97
AR	762	1.04	2.08	1.51
AP2	471	0.97	1.45	4.24
GR/PR	417	1.13	1.12	3.04

^{^a}

TF binding activity to haplotype 1 measured with chemiluminescence and expressed as RLU. RLU values are inversely correlated with the binding activities of TFs evaluated.

^{^b}

The change in binding activity is expressed as the ratio of RLU of the tested haplotype over RLU of haplotype 1. A ratio <1.0 indicates stronger TF promoter binding than to haplotype 1, whereas a ratio >1.0 indicates lower promoter binding for the TF than to haplotype 1.

RLU, relative light units; TF, transcription factor.