Figure 4.

Cellular uptake analyses in MKN45 cells.

Notes: The hydrophobic Coumarin 6 was used to mimic the existence of docetaxel. The same concentration of well-dispersed fluorescent NPs (0.125 mg/mL) and Coumarin 6-PEG-PCL-mAb NPs was applied for incubation with the PD-L1 plasmid transfected and untransfected MKN45 cells for 1 hour (A) and 2 hours (B) at 37°C. Scale bars are labeled on the figures. **P<0.01, and *P<0.05 were considered significant.

Abbreviations: mAb, monoclonal antibody; NP, nanoparticle; PD-L1, programmed death-ligand 1; PEG-PCL, poly (ethylene glycol)-poly (ε-caprolactone).