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. 2016 Jan 22;25(8):1524–1534. doi: 10.1016/j.sjbs.2016.01.031

Figure 2.

Figure 2

(a) Shows the effect of hexane extract of T. claveryi on nucleic morphology of U-87 MG cancer cells. Untreated and treated cells were stained with Hoechst 33342 to detect apoptotic morphology. (A) Control untreated cells. (B) Treated cells with 50 μg/ml of T. claveryi (C) Positive control, treated cells with 50 μg/ml of butanolic acid. Cells treated with the extract displayed characteristics of apoptosis with condensed and fragmented nuclei. Red arrows represent cells with DNA condensation. (b) Shows the Graphical percentage of apoptotic indices for U-87 MGcancer cells. The apoptotic index was expressed as a percentage ratio of number of apoptotic cells to the total number of cell in 10 different microscopic fields. Values are expressed as mean ± SD. ∗∗∗p < 0.001 compared to the control group.