Figure 6.

The clustering analysis of the profiles of transcripts in experiment 2. A hierarchical clustering analysis was done for the transcripts identified in experiment 2, following the same procedure as for experiment 1. The transcripts that were downregulated in the acute Cort (1) 1 h group outnumbered the upregulated ones. The upregulation of transcripts was short lived, and the expression levels of most transcripts, which were upregulated in the acute Cort (1) 1 h group compared with the ADX + Cort suppl group, recovered to the control levels in the acute Cort (1) 3 h group. The expression levels of most of the transcripts that were downregulated at 1 hour tended to recover at 3 hours, but most of them remained at lower levels than in the control. Fewer transcripts remained downregulated at 3 hours to the same degree as at 1 hour. A small number of transcripts, including Nab2, were “slow responders” and were upregulated at 3 hours but not at 1 hour. Note that the number of genes presented in the heatmap is slightly greater than that shown in Venn diagram (Fig. 4c) because of the clustering procedure (see “Materials and Methods”).