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. 2018 Oct 25;160(1):38–54. doi: 10.1210/en.2018-00535

Figure 9.

Figure 9.

Results of in situ hybridization to validate the changes in IEG mRNAs after acute Cort injection and to localize mRNA expression in the PVH. The effects of acute Cort injection on Fos and Egr1 mRNA expression were examined by semiquantitative in situ hybridization. (Upper panels) Autoradiograms show either Fos or Egr1 mRNA could hardly be observed in the PVH in the acute vehicle 1 h group. However, Fos and Egr1 mRNA levels became increased marked and observed clearly in the acute Cort (1) 1 h group. Fos and Egr1 mRNA were localized clearly in the PVH. (Lower panels) Densitometry analysis. By quantification of the autoradiograms, Fos and Egr1 mRNA expression levels were significantly greater in the acute Cort (1) 1 h group than those in the acute vehicle 1 h group. In contrast, in the acute Cort (10) 1 h group, neither Fos nor Egr1 mRNA levels were affected significantly compared with those in the acute vehicle 1 h group. *P < 0.05. NS, not significant.