Fig 5. Performance indices measured at multiple times after conditioning CS flies at 20, 30, and 40 days of age compared to control 5-day-old flies.

(A) Female CS flies. The Performance Index, tested at 3 min after conditioning, was significantly decreased in 20-, 30- and 40-day-old flies. The Performance Index at 1 hour was significantly decreased in 30- and 40-day-old flies but not at 3 hours, consistent with results of [12]. This is likely due to the reduced sensitivity to extract real differences at low performance values. (B) Male CS flies. The Performance Index was significantly reduced in 30- and 40-day-old flies. The Performance Index at 1 hour was significantly reduced in 30-day-old flies but not at 3 hours.