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. 2018 Dec 21;13(12):e0209407. doi: 10.1371/journal.pone.0209407

Fig 10. CEP1 is expressed in the root epidermis cells at lateral root (LR) emergence sites.

Fig 10

The eight stages (roman numbers, see S6 Fig) of LR primordium development in the roots of 10 day old cep1 ko mutant seedlings expressing pro-EGFP-CEP1 under the control of the endogenous promoter (PCEP1::pre-pro-3xHA-EGFP-AtCEP1-KDEL) were analyzed in vivo by CLSM (Olympus Fluoview FV 1000). No EGFP-CEP1 expression could be observed from developmental stage I-VI. (A-D) Stage VII of LR primordium development. (E-H) Stage VIII of LR primordium development. (I-M) Stage LR exhibiting the adult lateral root in LR primordium development. Arrow head in A, B and C indicates the LR primordium just emerging and separating the epidermis cells. The asterisk in B, F and K marks the epidermis cell expressing pro-EGFP-CEP1 at LR emergence sites. A, E and I: Focus is on the LR primordium. B-D, F-H, K-M: Focus is on the epidermis cells. A, B, E, F, I, K: Differential interference contrast. D, H, M: EGFP; C, G, L: merge.