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. 2018 Dec 21;13(12):e0209407. doi: 10.1371/journal.pone.0209407

Fig 11. CEP2 is expressed in the root epidermis cells at lateral root (LR) emergence sites.

Fig 11

(A-C) The eight stages (roman numbers, see S6 Fig) of LR primordium development in the roots of 10 day old WT seedlings expressing the pro-mCherry-CEP2 reporter under the control of the endogenous promoter (PCEP2::pre-pro-3xHA-mCherry-AtCEP2-KDEL) were analyzed in vivo by CLSM (Leica SP8). (A) Stage I-VI of LR primordium development. (B) Stage VII of LR primordium development. (C) Stage VIII of LR primordium development. The white asterisk indicates epidermis cells expressing pro-mCherry-AtCEP2 for LR emergence. (D-F) CEP2 was immunolocalized to epidermis cells that have to be moved for the emerging LR. (D) Maximum projection of LR emergence; LR, lateral root; VC, vascular cylinder; E, epidermis. (E) Single optical section shows CEP2 accumulation only in the epidermal cell bordering the LR outbreak. (F) Magnification of epidermal cell next to LR outgrow.