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. 2018 Dec 21;13(12):e0209407. doi: 10.1371/journal.pone.0209407

Fig 12. Loss of function of CEP1 or CEP2 retards lateral root (LR) emergence.

Fig 12

The quantitative evaluation of the eight stages (roman numbers, see S5 Fig) of LR primordium development in the roots of 10 day old cep1 ko, cep2 ko, and cep1/2 double ko/kd mutants (cep1+cep2-RNAi) were analyzed and compared to WT plants 49 hours after initiating the LR formation by “root bending”, when most of the WT plant LR primordia just reached the epidermis (stage VII). Single loss of function results in the strongest quantitative phenotype in retardation of LR development as compared to WT. Double loss of function does not result in an enhanced retardation of LR emergence. Columns represent the respective means expressed as percentage of LR primordia in the respective developmental stage in four independent experiments (biological replica): WT (15, 20, 18, or 20 plants per biological replica), cep1 ko (26, 24, 30, or 27 plants), cep2 ko (25, 29, 26, or 28 plants), cep1/2 double ko/kd mutants (cep1+cep2-RNAi) (21, 24, 22, or 26 plants). Columns marked with different letters indicate statistically different groups according to the ANOVA-and Duncan test (p<0.05).