Fig 6. Effect of rTWEAK treatment on intracellular signaling in GO and non-GO cells.

Cells were treated with rTWEAK (10 ng/ml) for various times and cell lysates were analyzed by western blotting to assess p-ERK 1/2, p-Akt, p-PI3K, p-JNK, and p-NF-κB p65 levels, with β-actin serving as a loading control.