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. 2018 Dec 21;13(12):e0209583. doi: 10.1371/journal.pone.0209583

Fig 8. Effect of Fn14 inhibitor on rTWEAK-induced hyaluronan production in GO cells.

Fig 8

Hyaluronan (ng/ml) level in GO orbital fibroblasts (n = 3) was measured by ELISA after treatment with rTWEAK (10, 100 and 200 ng/ml) for 6 and 24 h. Cells (n = 3) were pretreated with ERK kinase (PD98059, 20 μM), JNK (SP600125, 20 μM), p38 MAPK (SB203580, 20 μM), NF-κB p65 (SC514, 10 μM), and Fn14 (ITEM4, 2.5 μg/ml) inhibitor for 1 h followed by rTWEAK treatment (100 ng/ml, 24 h). Data in columns represent mean ± standard deviation of three experiments. *P < 0.05 vs. rTWEAK-treated cells without inhibitor pretreatment.