Table 1. Comparison of the high-glycerol (HGM) and low-glycerol (LGM) methods for the cryopreservation of human RBCs.

CONDITIONS	HGM (~40–50% w/v)	LGM (~15–20% w/v)
Freezing Temperature	-80°C	-197°C
Freezing Rate	Slow (1C/min)	Fast
Freezing Technique	Mechanical freezer	Liquid nitrogen
Storage Temperature	Min. -65°C	Min. -120°C
Impact of temperature changes	Thawing/refreezing possible	Highly destructive
Transportation	Dry ice	Nitrogen vapor
Time to deglycerolize	Lengthy (60 min)	Moderate (30 min)
Typical lysis following thaw	1–5% [19–22]	2–20% [18, 23, 24]
Typical lysis following CPA removal	10–20% [19–23, 25–27]	5–13% [18, 23]