Skip to main content
. 2018 Dec 21;13(12):e0201270. doi: 10.1371/journal.pone.0201270

Fig 10. Binary Y2H validation of potential interaction partners of N-TPL.

Fig 10

Y2H analysis of N-TPL, fused to the DBD, and potential interaction partners, fused to the AD of the GAL4 TF (in the pDEST22 vector), grown on selective medium SD-Leu-Trp-His (-3). Co-transformed PJ69-4α yeast strains were also grown on SD-Leu-Trp (-2) medium to confirm growth capacity. No direct interaction was confirmed between ATCKA2 encoded by AT3G5000 and N-TPL, in contrast to the interactions with all other potential interactors selected from the list with a threshold of SNRNINJA/EMPTY>7.2 and FPKMNINJA<100 values. * indicates a truncated version of the protein, as it was present in the Y2H cDNA library.