Table 4. Signal–to-noise ratio of the FPKM values of TPL-N and EMPTY Y2H-seq screenings.

Genes with SNR_TPL-N/EMPTY>6 were retained, listed and ranked from high to low SNR. Flagged genes are italicized. Previously reported interactors of TPL are indicated in bold. Potential interactors that were tested for binary interaction in further validation assays are underlined. A ‘Y’ in bold font indicates the presence of an EAR domain in the wrong frame or in an untranslated region of the gene.

	Gene ID	Gene- length	FPKMEMPTY	FPKMN-TPL	SNRFPKM	Gene Alias	Full- length	EAR domain
	FPKMN-TPL>100
1	AT5G25890	873	11,301	3997,602	353,736	IAA28/IAR2	Y	Y
2	AT3G23030	941	49,339	8581,581	173,933	IAA2	Y	Y
3	AT5G43700	1168	0,994	111,604	112,307	ATAUX2-11/IAA4	Y	Y
4	AT4G28640	1202	20,761	1121,873	54,037	IAA11	Y	Y
5	AT4G29080	1337	4,341	219,498	50,568	IAA27/PAP2	Y	Y
6	AT3G15540	970	7,180	266,613	37,135	IAA19/MSG2	Y	Y
7	AT1G04250	1087	249,863	8692,277	34,788	AXR3/IAA17	Y	Y
8	AT3G50000	1467	19,384	543,833	28,055	ATCKA2	Y
9	AT2G33310	1820	64,093	1054,613	16,454	IAA13	Y	Y
10	AT2G46990	655	9,746	158,039	16,215	IAA20	Y	Y
11	AT5G13790	962	15,685	198,181	12,635	AGL15	Y	Y
	FPKMN-TPL<100
12	AT3G54390	1341	3,0294	69,6555	22,9933		Y
13	AT4G37940	715	0,8117	15,1114	18,6177	AGL21	Y
14	AT2G40260	1233	0,4707	8,1976	17,4165		Y
16	AT3G58820	1391	0,4172	5,2619	12,6120			Y
17	AT1G51950	1539	4,9022	56,1646	11,4570	IAA18	Y	Y
18	AT1G04100	1254	1,3884	15,8426	11,4108	IAA10	Y	Y
19	AT3G05670	3090	0,9391	9,2492	9,8493			Y
20	AT4G31620	1809	0,9624	9,2481	9,6091			Y
21	AT2G33550	1194	8,2629	77,0636	9,3265			Y
22	AT1G08290	1760	0,3297	2,9705	9,0085	WIP3
23	AT3G19860	1288	3,1540	25,7074	8,1506	bHLH121	Y
24	AT5G25160	959	1,2103	9,4494	7,8074	ZFP3	Y	Y
25	AT5G47110	1088	1,0668	8,3290	7,8074	LIL3:2
26	AT3G04730	1279	0,4537	3,5426	7,8074	IAA16		Y
27	AT5G04550	2580	0,2249	1,7562	7,8074
28	AT3G15760	843	0,6884	4,9614	7,2068		Y
29	AT1G12270	1949	0,5955	4,2919	7,2068	Hop1
30	AT3G47980	1097	0,5290	3,8126	7,2068			Y
31	AT1G02650	1542	0,3764	2,7124	7,2068
32	AT2G38950	2482	0,2338	1,6851	7,2068
33	AT3G19070	1041	1,6725	11,7184	7,0066			Y
34	AT1G28300	1317	0,8813	6,0868	6,9066	AtLEC2		Y
35	AT3G56250	669	0,8675	5,7308	6,6063		Y
36	AT1G01030	1905	0,3046	2,0126	6,6063	NGA3		Y
37	AT1G61900	1913	0,3034	2,0041	6,6063			Y
38	AT1G79950	3123	0,1858	1,2276	6,6063
39	AT3G43575	4332	0,1340	0,8850	6,6063
40	AT5G36870	5616	0,2067	1,3033	6,3060	ATGSL09/atgsl9
41	AT2G30540	680	4,2672	26,6529	6,2459
42	AT2G38110	1845	4,0891	25,5028	6,2367	ATGPAT6		Y
43	AT2G25180	1980	3,2241	19,8913	6,1695	ARR12/AtARR12		Y
44	AT1G53030	530	1,0950	6,5762	6,0057
45	AT1G13680	1180	0,4918	2,9537	6,0057
46	AT4G19540	1215	0,4776	2,8686	6,0057	INDH/INDL	Y
47	AT3G56160	1600	0,3627	2,1784	6,0057
48	AT5G03570	1673	0,3469	2,0833	6,0057	ATIREG2/FPN2
49	AT3G59150	1866	0,3110	1,8678	6,0057		Y
50	AT4G03560	2674	0,2170	1,3034	6,0057	ATCCH1/ATTPC1/FOU2
51	AT3G03680	3308	0,1754	1,0536	6,0057