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. 2018 Nov 26;7:e38579. doi: 10.7554/eLife.38579

Figure 1. FoxA1 and FoxA2 are required for mucinous lung adenocarcinoma formation.

Photomicrographs of lung neoplasia arising 11 weeks after initiation with PGK-Cre lentivirus. All mice are KrasLSL-G12D/+; Nkx2-1F/F and harbor conditional alleles of Foxa1 and/or Foxa2 as indicated. (A) Hematoxylin and eosin (H and E) and immunohistochemistry (IHC) for NKX2-1, FoxA1 and FoxA2. Arrows indicate neoplasia lacking expression of all three proteins. Scale bar: 100 microns. (B) Alcian blue stain for mucin production. Scale bar: 50 microns. (C) IHC for markers of gastrointestinal differentiation HNF4α, PK-L and PDX1. Scale bar: 100 microns.

Figure 1.

Figure 1—figure supplement 1. FoxA1 and FoxA2 are required for mucinous lung adenocarcinoma formation.

Figure 1—figure supplement 1.

(A) IHC for indicated proteins on primary human mucinous lung adenocarcinoma. Images are representative of six independent primary tumors. Scale bar: 100 microns. (B) FOXA1 and FOXA2 mRNA levels in KRAS-mutant human lung adenocarcinoma and adjacent normal lung. Clusters were defined in Skoulidis et al. (2015) using TCGA data. HNF4α-positive/NKX2-1-negative tumors are found predominantly in KC cluster. (C) IHC for indicated proteins on lung neoplasia arising 11 weeks after initiation with PGK-Cre lentivirus. All mice are KrasLSL-G12D/+; Nkx2-1F/F and harbor conditional alleles of Foxa1 and/or Foxa2. Scale bar: 100 microns.