Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Goat polyclonal anti-Sox2 |
R + D Systems |
AF2018, RRID:AB_355110 |
(1:200) |
| Antibody | Goat polyclonal anti-Sox2 |
Santa Cruz Biotechnology |
sc-17320 RRID:AB_2286684 |
(1:200) |
| Antibody | Rabbit polyclonal anti-Tbr2 |
Abcam | ab23345, RRID:AB_778267 |
(1:250) |
| Antibody | Mouse monoclonal anti-PCNA |
Millipore | CBL407, RRID:AB_93501 |
(1:200) |
| Antibody | Mouse monoclonal anti-PhVim |
Abcam | ab22651, RRID:AB_447222 |
(1:200) |
| Antibody | Mouse monoclonal anti-Satb2 |
Abcam | ab51502, RRID:AB_882455 |
(1:200) |
| Antibody | Rabbit polyclonal anti-Tbr1 |
Abcam | ab31940, RRID:AB_2200219 |
(1:200) |
| Antibody | Rabbit polyclonal anti-Brn2 |
Proteintech | 18998–1-AP, RRID:AB_10597389 |
(1:100) |
| Antibody | Rabbit polyclonal anti-Olig2 |
Millipore | ab9610, RRID:AB_ 570666 |
(1:500) |
| Antibody | Rabbit polyclonal anti-GFAP |
Dako | Z0334, RRID:AB_10013382 |
(1:1000) |
| Antibody | Rabbit polyclonal anti-S100ß |
Abcam | ab868, RRID:AB_306716 |
(1:200) |
| Antibody | Rabbit polyclonal anti-active Caspase 3 |
Abcam | ab2302 RRID:AB_302962 |
(1:200) |
| Antibody | Mouse monoclonal anti-ARHGAP11B |
MPI-CBG | (1:100) | |
| Antibody | Rabbit polyclonal anti- Arhgap11A |
Abcam | ab113261, RRID:AB_ 10866587 |
(1:500) |
| Antibody | Chicken polyclonal anti-GFP |
Aves labs | GFP1020, RRID:AB_10000240 |
(1:1000) |
| Antibody | Goat polyclonal anti-GFP |
MPI-CBG | (1:1000) | |
| Antibody | Rat monoclonal anti-RFP |
ChromoTek | 5F8, RRID:AB _2336064 |
(1:500) |
| Antibody | Rabbit polyclonal anti-RFP |
Rockland antibodies |
600-401-379, RRID:AB_2209751 |
(1:1000) |
| Antibody | Goat polyclonal anti- Chicken Alexa Fluor 488 |
ThermoFisher Scientific |
A11039, RRID:AB_142924 |
(1:500) |
| Antibody | Donkey polyclonal anti- Goat Alexa Fluor 488 |
ThermoFisher Scientific |
A11055, RRID:AB_ 2534102 |
(1:500) |
| Antibody | Donkey polyclonal anti-Goat Alexa Fluor 555 |
ThermoFisher Scientific |
A21432, RRID:AB_2535853 |
(1:500) |
| Antibody | Donkey polyclonal anti-Goat Alexa Fluor 647 |
ThermoFisher Scientific |
A21447, RRID:AB_141844 |
(1:500) |
| Antibody | Donkey polyclonal anti-Rabbit Alexa Fluor 488 |
ThermoFisher Scientific |
A21206, RRID:AB_141708 |
(1:500) |
| Antibody | Donkey polyclonal anti-Rabbit Alexa Fluor 555 |
ThermoFisher Scientific |
A31572, RRID:AB_162543 |
(1:500) |
| Antibody | Donkey polyclonal anti-Rabbit Alexa Fluor 647 |
ThermoFisher Scientific |
A-31573, RRID:AB_2536183 |
(1:500) or (1:1000) |
| Antibody | Donkey polyclonal anti-Mouse Alexa Fluor 488 |
ThermoFisher Scientific |
A-21202, RRID:AB_141607 |
(1:500) or (`1:1000) |
| Antibody | Donkey polyclonal anti-Mouse Alexa Fluor 555 |
ThermoFisher Scientific |
A31570, RRID:AB_2536180 |
(1:500) |
| Antibody | Donkey polyclonal anti-Mouse Alexa Fluor 647 |
ThermoFisher Scientific |
A31571, RRID:AB_162542 |
(1:500) |
| Antibody | Goat polyclonal anti-Rat Alexa Fluor 555 |
ThermoFisher Scientific |
A21434, RRID:AB_ 141733 |
(1:500) or (1:1000) |
| Antibody | Goat polyclonal anti-Rabbit Cy2 |
Jackson Immuno research |
# 111-225-144, RRID:AB_2338021 |
(1:500) |
| Antibody | Goat polyclonal anti-Mouse Cy3 |
Jackson Immuno research |
#115-165-166, RRID:AB_2338692 |
(1:500) |
| Antibody | Goat polyclonal anti-Mouse Cy5 |
Jackson Immuno research |
#115-175-166, RRID:AB_2338714 |
(1:500) |
| Commercial assay or kit |
Maxi prep kit | Qiagen | Cat#12362 | |
| Commercial assay or kit |
RNeasy FFPE RNA isolation kit |
Qiagen | Cat# 73504 | |
| Commercial assay or kit |
NeuroTraceTM
640/660 deep-red fluorescent Nissl stain |
Molecular probes | Cat# N-21483, RRID:AB_2572212 |
|
| Commercial assay or kit |
In Situ Cell Death Detection Kit, TMR red (TUNEL) |
Sigma- Aldrich |
12156792910 | |
| Commercial assay or kit |
Annexin V Cy5 reagent |
Biovision | 1013–200 | |
| Commercial assay or kit |
Click-iT EdU Alexa Fluor 647 Imaging Kit |
Invitrogen | C10340 | |
| Strain, strain background (Mustela putorius furo) |
Ferret | Marshall Bioresources |
||
| Strain, strain background (Mustela putorius furo) |
Ferret | Euroferret | ||
| Sequence-based reagent |
Hprt1 FqHprt1_F
TACGCTGAGGATTTGGAAAAG |
This paper | oligonucleotide | |
| Sequence-based reagent |
Hprt1 FqHprt1_R
CCATCTCCTTCATCACGTCTC |
This paper | oligonucleotide | |
| Sequence-based reagent |
ARHGAP11B qARHGAP11B_1F
CAGAAAAGAAGGGCGTGTAC |
This paper | oligonucleotide | |
| Sequence-based reagent |
ARHGAP11B qARHGAP11B_1R
GGAGTAGCACAGAGACCATCA |
This paper | oligonucleotide | |
| Sequence-based reagent |
ARHGAP11B qARHGAP11B_2F
TGAGAATAAGATGGATAGCAGCA |
This paper | oligonucleotide | |
| Sequence-based reagent |
ARHGAP11B qARHGAP11B_2R
GGTACACGCCCTTCTTTTCTG |
This paper | oligonucleotide | |
| Recombinant DNA reagent |
pCAGGS | (Florio et al., 2015) | ||
| Recombinant DNA reagent |
pCAGGS-ARHGAP11B | (Florio et al., 2015) | ||
| Recombinant DNA reagent |
pCAGGS-mCherry | (Tavano et al., 2018) | ||
| Recombinant DNA reagent |
pCAGGS-GFP | (Fei et al., 2014) | ||
| Software, algorithm | Fiji/ImageJ | Fiji/Imagej | https://imagej.nih.gov/ij/ | |
| Software, algorithm | Prism | GraphPad software |
||
| Software, algorithm | ZEN | Carl Zeiss |