Table 1.

Parameters describing in vivo midazolam exposure and exosome CYP3A4 protein and mRNA expression

Parameter	Study phase	Geometric mean	95% confidence interval
			Lower	Upper
In vivo Midazolam AUC	Baseline	8.2	4.9	11.5
	Induced	2.2	1.5	2.9
In vivo Midazolam C max	Baseline	4.1	3.2	5.0
	Induced	0.9	0.6	1.2
Exosome CYP3A4 protein expression	Baseline	0.24	0.20	0.28
	Induced	0.42	0.21	0.65
Exosome CYP3A4 mRNA expression	Baseline	6.0	1.1	32.7
	Induced	48.3	11.3	104
Ex Vivo CYP3A4 activity	Baseline	25.8	22.9	30.5
	Induced	38.9	27.7	68.1

Area under the plasma–concentration time curve (AUC): μg l^–1 h; maximal concentration (C_max): μg l^–1; Exosome CYP3A4 protein expression; ng ml^–1 concentrated exosome sample, exosome CYP3A4 mRNA expression (x10^–11); 2^‐ΔΔCt, Ex vivo CYP3A4 activity (rate of 1‐hydroxy midazolam formation at 50 μmol l^–1 midazolam); pmol min^–1 mg^–1