| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse MRP14 (clone 2B10) | Gift from Dr N. Hogg (Cancer Research UK UK) (Hobbs et al., 2003) | NA |
| Anti-mouse α-SMA (clone 1A4) | Sigma-Aldrich | Cat#A5228; RRID: AB_262054 |
| Anti-mouse CD31 (clone 390) | Thermo Fisher Scientific | Cat#16-0311-85; RRID: AB_468933 |
| Anti-mouse VE-Cadherin (clone eBioBV14) | Thermo Fisher Scientific | Cat#14-1442-85; RRID: AB_891373 |
| Anti-mouse ACKR1 (clone 6B7) | (Thiriot et al., 2017) | NA |
| Anti-mouse PDGFR-β (polyclonal) | R&D Systems | Cat#AF1042; RRID: AB_2162633 |
| Anti-mouse NG2 (polyclonal) | Millipore | Cat#AB5320; RRID: AB_91789 |
| Blocking anti-mouse CXCL1 (clone 48415) | R&D Systems | Cat#MAB453; RRID: AB_2087696 |
| Blocking anti-mouse CXCL2 (clone 40605) | R&D Systems | Cat#MAB452; RRID: AB_2230058 |
| Anti-mouse CXCL1 (polyclonal) | R&D Systems | Cat#AF-453-NA; RRID: AB_354495 |
| Anti-mouse CXCL2 (polyclonal) | R&D Systems | Cat#AF-452-NA; RRID: AB_2086326 |
| Blocking anti-mouse CXCL5 (clone 61905) | R&D Systems | Cat#MAB433; RRID: AB_2086587 |
| Blocking anti-mouse MIF (clone CT 1C10) | Gift from Dr C. Weber (Ludwig Maximilians University Munich, Germany) | NA |
| Blocking anti-mouse CXCR2 (clone 242216) | R&D Systems | Cat#MAB2164; RRID: AB_358062 |
| Alexa Fluor 647 anti-mouse CXCR2 (clone SA044G4) | Biolegend | Cat#149306; RRID: AB_2565694 |
| Pacific Blue anti-mouse CD45 (clone 30-F11) | Biolegend | Cat#103126; RRID: AB_493535 |
| Alexa Fluor 647 anti-mouse Ly6G (clone 1A8) | Biolegend | Cat#127610; RRID: AB_1134159 |
| PE anti-mouse CD115 (clone AFS98) | Thermo Fisher Scientific | Cat#12-1152-83: RRID: AB_465809 |
| Anti-pan-AKT (polyclonal) | Cell Signaling Technology | Cat#9272; RRID: AB_329827 |
| Anti-phospho-AKT (clone D9E) | Cell Signaling Technology | Cat#4060; RRID: AB_2315049 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BSA, low endotoxin | Sigma-Aldrich | Cat#A9543 |
| Bovine collagen type I | Advanced Biomatrix | Cat#5005 |
| DMEM, low glucose | Thermo Fisher Scientific | Cat#11885084 |
| Fluo-4, AM, cell permeant | Thermo Fisher Scientific | Cat#F14201 |
| HALT protease and phosphatase inhibitor cocktail | Thermo Fisher Scientific | Cat#78440 |
| LPS | Sigma-Aldrich | Cat#L4391 |
| LTB4 | Cayman Chemical | Cat#20110 |
| Pluronic F-127 | Thermo Fisher Scientific | Cat#P3000MP |
| Probenecid, water soluble | Thermo Fisher Scientific | Cat#P36400 |
| Recombinant human PEDF | Sigma-Aldrich | Cat#SRP4988 |
| Recombinant murine CXCL1 | Preprotech | Cat#250-11 |
| Recombinant murine CXCL2 | Preprotech | Cat#250-15 |
| Recombinant human ICAM-1 | R&D Systems | Cat#ADP4-050 |
| Recombinant murine IL-1β | R&D Systems | Cat#401-ML-005/CF |
| Recombinant murine TNF-α aa 80-235 | R&D Systems | Cat#410-MT-010/CF |
| 3,3′,5,5′-tetramethylbenzidine substrate solution | Thermo Fisher Scientific | Cat#00-4201-56 |
| Critical Commercial Assays | ||
| Alexa Fluor 488 antibody labeling kit | Thermo Fisher Scientific | Cat# A20181 |
| Alexa Fluor 555 antibody labeling kit | Thermo Fisher Scientific | Cat#A20187 |
| Alexa Fluor 647 antibody labeling kit | Thermo Fisher Scientific | Cat#A20186 |
| DyLight 405 antibody labeling kit | Thermo Fisher Scientific | Cat#53021 |
| iQ SYBR Green supermix | Biorad | Cat#1708880 |
| Neutrophil isolation kit (mouse) | Miltenyi Biotec | Cat#130-097-658 |
| Quantikine ELISA mouse CXCL1 | R&D Systems | Cat#MKC00B |
| Quantikine ELISA mouse CXCL2 | R&D Systems | Cat#MM200 |
| RNAscope fluorescent multiplex assay | Advanced Cell Diagnostics | Cat#320851 |
| Experimental Models: Organisms/Strains | ||
| Mouse, Ackr1−/− | (Dawson et al., 2000) | NA |
| Mouse, Acta2-RFPcherry-Tg | Gift from Dr D. Rowe (University of Connecticut Health Center, US) (Proebstl et al., 2012) | NA |
| Mouse, Lyz2-EGFP-ki;Acta2-RFPcherry-Tg | (Proebstl et al., 2012) | NA |
| Mouse, Cxcl2−/− | The Jackson Laboratory | JAX 029557 |
| Mouse, Cxcr2−/− | (Cacalano et al., 1994) | NA |
| Mouse, C57BL/6 | Charles River Laboratories | JAX 000664 |
| Mouse, Lyz2-EGFP-ki | Gift from Dr M. Sperandio (Ludwig Maximilians University Munich, Germany) (Faust et al., 2000) | NA |
| Oligonucleotides | ||
| mRNA probe: Pecam1-C3 | Advanced Cell Diagnostics | Cat#316721-C3 |
| mRNA probe: Acta2-C2 | Advanced Cell Diagnostics | Cat#319531-C2 |
| mRNA probe: Cxcl1-C1 | Advanced Cell Diagnostics | Cat#407721 |
| mRNA probe: Cxcl2-C1 | Advanced Cell Diagnostics | Cat#437581 |
| Real-time PCR primer: Gapdh forward 5′-TCGTGGATCTGACGTGCCGCCTG-3′ |
This paper | NA |
| Real-time PCR primer: Gapdh reverse 5′-CACCACCCTGTTGCTGTAGCCGTA-3′ |
This paper | NA |
| Real-time PCR primer: Cxcl1 forward 5′-CCGAAGTCATAGCCACACTCAA-3′ |
This paper | NA |
| Real-time PCR primer: Cxcl1 reverse 5′-GCAGTCTGTCTTCTTTCTCCGTTA-3′ |
This paper | NA |
| Real-time PCR primer: Cxcl2 forward 5′-GAAGTCATAGCCACTCTCAAGG-3′ |
This paper | NA |
| Real-time PCR primer: Cxcl2 reverse 5′-CCTCCTTTCCAGGTCAGTTAGC-3′ |
This paper | NA |
| Software and Algorithms | ||
| Chemotaxis and migration tool v2.0 | IBIDI | https://ibidi.com/manual-image-analysis/171-chemotaxis-and-migration-tool.html |
| FlowJo v10.2 | Tree Star | https://www.flowjo.com/ |
| ImageJ 1.49 | NIH | https://imagej.nih.gov/ij/ |
| Imaris v8 | Bitplane | http://www.bitplane.com/imaris |
| Prism v6.05 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Corning Transwell inserts | Sigma-Aldrich | Cat#CLS3415-48EA |
| 123count eBeads™ counting beads | Thermo Fisher Scientific | Cat#01-1234-42 |
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