Figure 2.

Tissue-Specific Oscillations in Endothelial Cell Adhesion Molecules

(A) Map of rhythmic protein expression of endothelial cell-adhesion molecules of various organs (n = 3–6 mice with 6 time points measured each; one-way ANOVA).

(B) Integration of all expressed molecules over all organs across the day (n = 3–6 mice with 6 time points measured each; one-way ANOVA).

(C) Integration of ICAM-1 and VCAM-1 expression over all organs across the day (n = 3–6 mice with 6 time points measured each; one-way ANOVA).

(D) Adoptive transfer of donor cells to recipients treated with functional blocking antibodies directed against the indicated molecules at ZT1 and ZT13. Results are presented as percentages of injected cells (n = 4–12 mice; one-way ANOVA followed by Dunnett comparison to control groups and unpaired Student’s t test for comparisons between ZT1 and ZT13 groups).

(E) Fold change of donor cells remaining in recipient blood at ZT1 and ZT13 after anti-VCAM-1 or anti-ICAM-1 antibody treatment, respectively, in comparison with numbers of isotype antibody controls (n = 7–11 mice; unpaired Student’s t test).

(F) Adoptive transfer of donor cells to Icam1^−/− recipients at ZT1 and ZT13 (n = 6–8 mice; unpaired Student’s t test).

(G) Endogenous blood leukocyte numbers in Icam1^−/− mice at ZT1 and ZT13 (n = 6–8 mice; unpaired Student’s t test).

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; ^{#, ##, ###, ####} indicate significance levels analogous to those of control groups. All data are represented as mean ± SEM. ns, not significant; MFI, mean fluorescence intensity. See also Figure S2 and Table S1.