REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD3, PE/DZL594, clone 17A2	Biolegend	Cat# 100246, RRID: AB_2565883
Anti-mouse CD3ε, Alexa Fluor® 488, clone 145-2C11	Biolegend	Cat# 100321, RRID: AB_389300
Anti-mouse CD4, Brilliant Violet 570, clone RM4-5	Biolegend	Cat# 100542, RRID: AB_2563051
Anti-mouse CD4, PE, clone GK1.5	Biolegend	Cat# 100408, RRID: AB_312693
Anti-mouse CD4, APC, clone GK1.5	Biolegend	Cat# 100412, RRID: AB_312697
Anti-mouse CD4, APC/Cy7, clone GK1.5	Biolegend	Cat# 100714, RRID: AB_312753
Anti-mouse CD8a, PE/Cy7, clone 53-6.7	Biolegend	Cat# 100722, RRID: AB_100722
Anti-mouse CD8a, APC/Cy7, clone 53-6.7	Biolegend	Cat# 100714, RRID: AB_312753
Anti-mouse CD8a, PE-CF594, clone 53-6.7	BD Bioscience	Cat# 562283
Anti-mouse CD8a, Alexa Fluor® 700, clone 53-6.7	Biolegend	Cat# 100730, RRID: AB_493703
Anti-mouse/human CD11b, Alexa Fluor® 700, clone M1/70	Biolegend	Cat# 101222, RRID: AB_493705
Anti-mouse CD115, PE, clone AFS98	Biolegend	Cat# 135506, RRID: AB_1937253
Anti-mouse CD115, APC, clone AFS98	eBioscience	Cat# 135510, RRID: AB_2085221
Anti-mouse Gr-1, PerCP/Cy5.5, clone RB6-8C5	Biolegend	Cat# 108428, RRID: AB_893558
Anti-mouse Gr-1, FITC, clone RB6-8C5	Biolegend	Cat# 108406, RRID: AB_313371
Anti-mouse/human CD45R/B220, Alexa Fluor® 488, clone RA3-6B2	Biolegend	Cat# 103225, RRID: AB_389308
Anti-mouse/human CD45R/B220, PE/Cy7, clone RA3-6B2	Biolegend	Cat# 103222, RRID: AB_313005
Anti-mouse/human CD45R/B220, APC-Cy7, clone RA3-6B2	Biolegend	Cat# 103224, RRID: AB_313007
Anti-mouse Siglec-F, Alexa Fluor® 647, clone E50-2440	BD Bioscience	Cat# 562680
Anti-mouse Siglec-F, APC-Cy7, clone E50-2440	BD Bioscience	Cat# 565527
Anti-mouse NK1.1, APC, clone PK136	Biolegend	Cat# 108710, RRID: AB_313397
Anti-mouse NK1.1, Alexa Fluor® 700, clone PK136	ebioscience	Cat# 56-5941-80, RRID: AB_2574504
Anti-mouse NK1.1, PE/Cy7, clone PK136	Biolegend	Cat# 108714, RRID: AB_389364
Anti-mouse NK1.1, PE/Cy5, clone PK136	Biolegend	Cat# 108716, RRID: AB_493590
Anti-mouse CD45, PE/Dazzle 594, clone 30-F11	Biolegend	Cat# 103146, RRID: AB_2564003
Anti-mouse CD45, PE, clone 30-F11	Biolegend	Cat# 103106, RRID: AB_312971
Anti-mouse CD45, APC, clone I3/2.3	Biolegend	Cat# 147708, RRID: AB_2563540
Anti-mouse CD45.1, PE-CF594, clone A20	BD Biosciences	Cat# 562452
Anti-mouse CD45.2, Alexa Fluor® 700, clone 104	Biolegend	Cat#109822, RRID: AB_493731
Anti-mouse CD45, eFluor780, clone 30-F11	eBiosciences	Cat# 47-0451-82, RRID: AB_1548781
Anti-mouse Ly6C, PE, clone HK1.4	Biolegend	Cat# 128008, RRID: AB_1186132
Anti-mouse Ly6G, PerCP/Cy5.5, clone 1A8	Biolegend	Cat# 127616, RRID: AB_1877271
Anti-mouse I-A/I-E, PE/Cy7, clone M5/114.15.2	Biolegend	Cat# 107630, RRID: AB_2069376
Anti-mouse CCR1, PE, clone 643854	R&D	Cat# FAB5986P-100
Anti-mouse CCR2, Alexa Fluor® 700, clone 475301	R&D	Cat# FAB5538N-100
Anti-mouse CCR3, FITC, clone J073E5	Biolegend	Cat# 144510, RRID: AB_2561609
Anti-mouse CCR3, PE, clone J073E5	Biolegend	Cat# 144506, RRID: AB_2561534
Anti-mouse CCR4, PE/Cy7, clone 2G12	Biolegend	Cat# 131214, RRID: AB_2244410
Anti-mouse CCR5, PE, clone HM-CCR5	Biolegend	Cat# 107006, RRID: AB_313301
Anti-mouse CCR6, PerCp/Cy5.5, clone 29-2L17	Biolegend	Cat# 129810, RRID: AB_2275515
Anti-mouse CCR7, PE, clone 4B12	Biolegend	Cat# 120106, RRID: AB_389358
Anti-mouse CCR8, PE, clone 1055c	R&D	Cat# FAB8324P-100
Anti-mouse CCR9, PE, clone 9B1	Biolegend	Cat# 129708, RRID: AB_2073249
Anti-mouse CCR10, Alexa Fluor® 700, clone 248918	R&D	Cat# FAB2815N-100
Anti-mouse CXCR2, PE, clone 242216	R&D	Cat# FAB2164P-100
Anti-mouse CXCR3, PE/Cy7, clone CXCR3-173	Biolegend	Cat# 126516, RRID: AB_2245493
Anti-mouse CXCR4, PE, clone L276F12	Biolegend	Cat# 146506, RRID: AB_2562783
Anti-mouse CXCR4, PerCP/Cy5.5, clone L276F12	Biolegend	Cat# 146510, RRID: AB_2562787
Anti-mouse CXCR5, PE, clone L138D7	Biolegend	Cat# 145504, RRID: AB_2561968
Anti-mouse CXCR6, PerCP, clone 221002	R&D	Cat# FAB2145C-100
Anti-mouse CX3CR1, PerCP Cy 5.5, clone SA011F11	Biolegend	Cat# 149010, RRID: AB_2564494
Anti-mouse CD11a, PerCP/Cy5.5, clone M17/4	Biolegend	Cat# 101124, RRID: AB_2562932
Anti-mouse/human CD11b, Alexa Fluor® 700, clone M1/70	Biolegend	Cat# 101222, RRID: AB_493705
Anti-mouse CD11c, Alexa Fluor® 700, clone N418	Biolegend	Cat# 117320, RRID: AB_528736
Anti-mouse CD44, PE-CF594, clone IM7	BD Bioscience	Cat# 562464
Anti-mouse CD62L, APC/Cy7, clone MEL-14	Biolegend	Cat# 104428, RRID: AB_830799
Anti-mouse CD162, PE, clone 2PH1	BD bioscience	Cat# 555306
Anti-mouse CD18, PerCP-Cy5.5, clone C71/16	BD Bioscience	Cat# 562827
Anti-mouse/rat CD29, Alexa Fluor® 700, clone HMβ1-1	Biolegend	Cat# 102218, RRID: AB_493711
Anti-mouse CD49b, PE/Cy7, clone DX5	Biolegend	Cat# 108922, RRID: AB_2561460
Anti-mouse CD49d, PerCP/Cy5.5, clone R1-2	Biolegend	Cat# 103620, RRID: AB_2563702
Anti-mouse CD49e, PE-CF594, clone 5H10-27 (MFR5)	BD Bioscience	Cat# 564313
Anti-mouse/human CD49f, PE/Cy7, clone GoH3	Biolegend	Cat# 313622, RRID: AB_2561705
Anti-human CD8, Alexa Fluor® 488, clone HIT8a	Biolegend	Cat# 300916, RRID: AB_756152
Anti-human CD19, PE, clone HIB19	Biolegend	Cat# 302208, RRID: AB_314238
Anti-human CD56, PE/Dazzle 594, clone HCD56	Biolegend	Cat# 318348, RRID: AB_2563564
Anti-human CD14, PerCP/Cy5.5, clone HCD14	Biolegend	Cat# 325622, RRID: AB_893250
Anti-human CD16, PE/Cy7, clone 3G8	Biolegend	Cat# 302016, RRID: AB_314216
Anti-human CD49d, APC, clone 9F10	Biolegend	Cat# 304308, RRID: AB_2130041
Anti-human CD4, Alexa Fluor® 700, clone SK3	Biolegend	Cat# 344622, RRID: AB_2563150
Anti-human CXCR4, APC/Cy7, clone 12G5	Biolegend	Cat# 306528, RRID: AB_2565994
Anti-human CD3, Brilliant Violet 570, clone UCHT1	Biolegend	Cat# 300436, RRID: AB_2562124
Anti-human CD45, PE, clone HI30	BD PharMingen	Cat# 555483, RRID: AB_395875
Anti-mouse Armenian Hamster IgG, Alexa Fluor® 700, clone HTK888	Biolegend	Cat# 400926
Anti-mouse Armenian Hamster IgG, PE, clone HTK888	Biolegend	Cat# 400908
Anti-mouse Armenian Hamster IgG, Pe/Cy7, clone HTK888	Biolegend	Cat# 400922
Anti-mouse Armenian Hamster IgG, PerCp/Cy5.5, clone HTK888	Biolegend	Cat# 400932
Anti-mouse Mouse IgG 1, κ, PE, clone P3.6.2.8.1	eBioscience	Cat# 12-4714-41
Anti-mouse Rat IgG1, κ, PE, clone R3-34	BD bioscience	Cat# 553925
Anti-mouse Rat IgG2a, κ, Alexa Fluor® 488, clone RTK2758	Biolegend	Cat# 400525
Anti-mouse Rat IgG2a, κ, Alexa Fluor® 700, clone eBR2a	eBioscience	Cat# 56-4321-80
Anti-mouse Rat IgG2a, k, APC/Cy7, clone RTK2758	Biolegend	Cat# 400524
Anti-mouse Rat IgG2a, κ, PE, clone RTK2758	Biolegend	Cat# 400508
Anti-mouse Rat IgG2a, κ, PE-CF594, clone R35-95	BD Bioscience	Cat# 562302
Anti-mouse Rat IgG2a, κ, PE/Cy7, clone RTK2758	Biolegend	Cat# 400522
Anti-mouse Rat IgG2a, κ, PerCp/Cy5.5, clone RTK2758	Biolegend	Cat# 400532
Anti-mouse Mouse IgG2a, κ, PerCp/Cy5.5, clone MOPC-173	Biolegend	Cat# 400258
Anti-mouse Rat IgG2b, κ, Alexa Fluor® 700, clone RTK4530	Biolegend	Cat# 400628
Anti-mouse Rat IgG2b, κ, PE, clone RTK4530	Biolegend	Cat# 400610
Anti-mouse Rat IgG2b, κ, PE/Cy7, clone RTK4530	Biolegend	Cat# 400618
Anti-mouse Rat IgG2b, κ, PE CP594, clone A95-1	BD Bioscience	Cat# 562308
Anti-mouse Rat IgG2b, κ, PerCP/Cy5.5, clone RTK4530	Biolegend	Cat# 400632
Anti-mouse ICAM-1, PE, clone YN1/1.7.4	Biolegend	Cat# 116108, RRID: AB_313699
Anti-mouse VCAM-1, PE, clone 429 (MVCAM.A)	Biolegend	Cat# 105714, RRID: AB_1134164
Anti-mouse E-selectin, PE, clone 10E9.6 (RUO)	BD Bioscience	Cat# 553751
Anti-mouse/human P-selectin, PE, clone Psel.KO2.3	eBioscience	Cat# 2-0626-80, RRID: AB_1210864
Anti-mouse MadCAM, Alexa Fluor® 488, clone MECA-367	Biolegend	Cat# 120708, RRID: AB_493398
Anti-mouse ICAM-2, Alexa Fluor® 488, clone 3C4 (MIC2/4)	Biolegend	Cat# 105609, RRID: AB_2264501
Anti-mouse/human PNAd, Biotin, clone MECA-79	Biolegend	Cat# 120804, RRID: AB_493557
Anti-mouse PECAM-1, Alexa Fluor® 647, clone MEC13.3	Biolegend	Cat# 102516, RRID: AB_2161029
Anti-mouse PECAM-1, APC, clone 390	Biolegend	Cat# 102410, RRID: AB_312905
Anti-mouse/human CD44, PE, clone IM7	Biolegend	Cat# 103008, RRID: AB_312959
Streptavidin, Cy3	Biolegend	Cat# 405215
Anti-mouse Rat IgM, κ, Biotin, clone RTK2118	Biolegend	Cat# 400804
Anti-mouse Rat IgG2a, κ, PE, clone RTK2758	Biolegend	Cat# 400508
Anti-mouse Rat IgG2b, κ, PE, clone RTK4530	Biolegend	Cat# 400608
Anti-mouse Rat IgG2a, κ, Alexa Fluor® 488, clone RTK2758	Biolegend	Cat# 400525
Anti-mouse Rat IgG1, κ, PE, clone P3.6.2.8.1	eBioscience	Cat# 12-4714-82
Anti-mouse anti-ICAM-1, clone YN1/1.7.4	BioXcell	Cat# BE0020-1, RRID: AB_1107661
Anti-mouse anti-ICAM-2, clone 3C4(mIC2/4)	BD bioscience	Cat# 553325
Anti-mouse anti-VCAM-1, clone M/K-2.7	BioXcell	Cat# BE0027, RRID: AB_1107572
Anti-mouse anti-P-selectin, clone RB40.34	BD bioscience	Cat# 553742
Anti-mouse anti-CD62E, clone 10E9.6	BD bioscience	Cat# 553749
Anti-mouse anti-CD62L, clone Mel-14	BioXcell	Cat# BE0021, RRID: AB_1107665
Anti-mouse anti-PSGL-1, clone 4RA10	BioXcell	Cat# BE0186, RRID: AB_10950305
Anti-mouse anti-CD18, clone M18/2	BioXcell	Cat# BE0009, RRID: AB_1107607
Anti-mouse anti-CD29, clone KMI6	BioXcell	Cat# BE0232, RRID: AB_2687714
Anti-mouse anti-CD49d, clone clone PS/2	BioXcell	Cat# BE0071, RRID: AB_1107657
Anti-mouse anti-CD11a, clone Clone: M17/4	BioXcell	Cat# BE0006, RRID: AB_1107578
Anti-mouse Rat IgG2a Isotype control, clone 2A3	BioXcell	Cat# BE0089, RRID: AB_1107769
Anti-mouse Rat IgG1 Isotype control, clone HRPN	BioXcell	Cat# BE0088, RRID: AB_1107775
Anti-mouse Rat IgG2b Isotype control, clone LTF-2	BioXcell	Cat# BE0090, RRID: AB_1107780
Chemicals, Peptides, and Recombinant Proteins
CCR4 antagonist, C 021 dihydrochloride	Tocris	Cat# 3581
CXCR2 antagonist, SB 265610	Tocris	Cat# 2724
CXCR4 antagonist, AMD 3100 octahydrochloride	Tocris	Cat# 3299
CellTrace CFSE	Thermo Fisher Scientific	Cat# C34554
Cell tracker Deep red	Thermo Fisher Scientific	Cat# C34565
Lipopolysaccharides (LPS)	Sigma	Cat# L4516
Tween80	Sigma	Cat# P4780
Dimethyl sulfoxide (DMSO)	Sigma	Cat# D2650
Collagenase IV	Sigma	Cat# C5138
Deoxyribonuclease I (Dnase I)	Aplicem	Cat# A3778
DAPI	Biolegend	Cat# 422801
Tamoxifen	Sigma	Cat# T5648
TNFα	Peprotech	Cat# 300-01A
IFNγ	Peprotech	Cat# 300-02
CXCL12	MerckSerono	In-house production
Critical Commercial Assays
EasySep mouse neutrophil enrichment Kit	STEMCELL Technologies	Cat# 19762
EasySep mouse monocyte isolation Kit	STEMCELL Technologies	Cat# 19861
EasySep mouse B cell isolation kit	STEMCELL Technologies	Cat# 19854
RNeasy Plus mini Kit	QIAGEN Hilden Germany	Cat# 74136
Human B cell negative selection kit	Miltenyi Biotec	Cat# 130-091-151
Experimental Models: Cell Lines
BS50 B-ALL cells	Rudi W Hendriks, Erasmus MC Rotterdam, the Netherlands	N/A
C1498 AML cells	ATCC	Cat# TIB49
NALM-6 human B-ALL cells	ATCC	Cat# CRL-3273
human umbilical vein endothelial cells	University Geneva Hospital	N/A
Experimental Models: Organisms/Strains
CD19-cre mice	Jackson Laboratories	Cat# 006785
Lyz2-cre mice	Jackson Laboratories	Cat# 004781
Bmal1flox/flox	Jackson Laboratories	Cat# 007668
Cdh5-cre/ERT2 mice	Ralf Adams, MPI Münster, Germany	N/A
C57BL/6J CD45.1 (B6.SJL-PtprcaPepcb/BoyCrl)	Charles River	Ly5.1 mice
NSG (NOD.Cg-PrkdcscidIL2rgtm1Wjl/SzJ)	Charles River	JAX Cat# 05557
Oligonucleotides
Nr1d1 Forward GAT AGC TCC CCT TCT TCT GCA TCA TC	Eurofins Genomics	N/A
Nr1d1 Reverse TTC CAT GGC CAC TTG TAG ACT TC	Eurofins Genomics	N/A
Sell Forward GAC GCC TGT CAC AAA CGA AA	Eurofins Genomics	N/A
Sell Reverse GCC CGT AAT ACC CTG CAT CA	Eurofins Genomics	N/A
Cxcl12 Forward CAG AGC CAA CGT CAA GCA	Eurofins Genomics	N/A
Cxcl12 Reverse AGG TAC TCT TGG ATC CAC	Eurofins Genomics	N/A
Software and Algorithms
GraphPad Prism7	Graphpad software	N/A
Flowjo 10.4	Flowjo, LLC	www.flowjo.com
Becton Dickinson	FACSDiva v8.0.1	N/A
Zeiss	ZEN	N/A
ImageJ version 1.51n	https://imagej.net	N/A
slidebook version 6	Intelligent Imaging Innovations, 3i	www.intelligent-imaging.com