Figure 6.

Biochemical characterization of N-cadherin (green; A and B) and vinculin (green; C and D) at the CM-CMF boundary on soft, moderate, and stiff samples. CMs were stained with cardiac troponin-T (purple), and cell nuclei were counterstained with DAPI. The fluorescence intensity was normalized to the total number of cells on each substrate and then averaged over three samples. The yellow arrows show the distribution of vinculin at the CM-CMF interface (scale bars, 50 μm). Data are shown as mean, and the error bar represents the SD of the measured values (^∗ indicates a statistically significant difference with p < 0.05). To see this figure in color, go online.