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RII phosphatase activity assay analyses for calcineurin and CaN∆442 in the absence and presence of CaM
| −CaM | +CaM | |||
|---|---|---|---|---|
| Km (μM) | Vmax (μmol min−1 mg−1) | Km (μM) | Vmax (μmol min−1 mg−1) | |
| CaN | – | – | 53 ± 8 | 0.9 ± 0.1 |
| CaN∆442 | 81 ± 0.3 | 1.1 ± 0.4 | 59 ± 16 | 1.2 ± 0.2 |
