Fig. 5.
CIR rich CD4+ T cells have a distinct transcriptional program. a Volcano plots of bulk RNA sequencing data of indicated populations (IL-10pos CIR rich n = 2; IL-10pos CIR poor n = 2; IL-10neg n = 2). b Expression of known TR1 signature genes comparing IL-10pos CIR rich with IL-10neg and IL-10pos CIR rich with IL-10pos CIR poor. c Differentially expressed transcription factors between IL-10pos CIR rich and IL-10pos CIR poor. mRNA expression of indicated genes normalized to Hprt from at least three independent experiments (separated low-high, line represents median). d CD4+ T cells isolated from Lxrα-/- CD45.2 and wildtype CD45.2 mice were co-transferred with wildtype CD4+ T cells (CD45.1/2) into lymphopenic hosts. Animals (WT:KO n = 7; WT:WT n = 6) were treated with aCD3 antibodies 5 weeks upon transfer and cells were isolated from small intestine. Results are cumulative of two independent experiments. A Wilcoxon test was used to calculate significance. e Correlation between genes significantly higher expressed in IL-10pos CIR rich versus IL-10pos CIR poor cells and expression pattern of scRNA-seq data