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. 2018 Dec 21;3:23. doi: 10.1038/s41536-018-0061-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Extracellular matrix levels in the cornea. Representative images of immunohistochemical staining with accompanying plots quantifying the IR for: a αSMA⁺ (green to stain myofibroblasts), b IR fibronectin⁺ (green to stain fibronectin in the ECM), and c laminin⁺ (red to stain laminin in the ECM), in each case DAPI⁺ was used to stain the cell nuclei (blue). Analysis was undertaken on intact eyes, eyes taken at 2 days post infection and eyes obtained after 16 days with various eye drop treatments: (i) Gentamicin and Prednisolone (G.P), (ii) Gentamicin, Prednisolone, and fluid gel (G.P.FG), and (iii) Gentamicin, Prednisolone and hrDecorin fluid gel (G.P.DecFG). All studies were done using n = 6 treatment groups, with quantification performed on masked images unknown to the observer (scale bar = 100 µm)