Skip to main content
. 2018 Dec 21;8:18038. doi: 10.1038/s41598-018-36061-4

Figure 2.

Figure 2

emx1 deficient embryos exhibit a DE expansion, DL reduction and an expansion of the associated CS gland. (A) WISH analysis (lateral view) of 28 ss WT, emx1 deficient, and emx1 deficient embryos co-injected with emx1 mRNA (emx1 rescue) for the distal segment markers (purple): slc12a1 for the DE, and slc12a3 for the DL. Blue boxes demarcate areas of pronephros gene expression. Scale bar = 100 μm. (B,C) Average segment lengths of the DE and DL for WT, emx1 deficient, and emx1 rescue embryos. (D) WISH panel (dorsal view) of 28 ss WT, emx1 deficient, and emx1 rescue embryos for the CS marker stc1 (purple). Blue boxes demarcate a single CS territory while the contralateral side is unlabeled. Scale bar = 20 μm. Quantification of stc1+ cell number (E) and size (F) of WT, emx1 deficient, and rescue embryos. ***p < 0.001; N.S. = not significant. Error bars indicate standard error. (G) Summary of pronephros phenotype in emx1 deficient embryos with respect to the axial location of somites.