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. 2018 Dec 21;8:18038. doi: 10.1038/s41598-018-36061-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial arrangement and size of renal cells in the DE and DL are unaffected by emx1 deficiency. (A,C) Confocal imaging of FISH analysis (lateral view) of 28 ss WT and emx1 deficient embryos for the DE and DL segment makers slc12a1 and slc12a3 (red), respectively, with DAPI staining (grey). Yellow lines demarcate segment domains. Scale bar = 20 μm. (B,D) Quantification of internuclear distance of DE and DL segment cells in WT and emx1 deficient embryos. Quantification of tubule volume (E), segment volume to segment length ratio (F), and the number of nuclei per 30 μm of segment in WT and emx1 deficient DE and DL segments (G). *<0.05; N.S. = not significant. Error bars represent standard error.