Fig. 2.

Characterization of CRISPR/Cas9-mediated Hao1 gene editing. Deep sequencing was performed 1 month after treatment on the DNA from livers of 12–14-week-old PH1 animals treated with Hao1-g1 (n = 5) and Hao1-g2 (n = 5), as well as Cas9 (n = 3) and saline (n = 1). a Frequency of CRISPR/Cas9 introduced variants in the Hao1 gene of individual animals. b Characterization of the variants according to their type, size, and frameshift potential of each animal treated with Hao1-g1 and Hao1-g2. a, b Each bar represents an individual mouse. c Frequency distribution of indel size in base pairs (bp) for each animal treated with Hao1-g1 (green lines) and Hao1-g2 (orange lines). Each line represents an individual mouse