Fig. 4.
The IFNα/βBP cell surface binding activity contributes to poxvirus pathogenesis. Balb/c mice (n = 5) were infected i.n. or s.c. in the footpad with the indicated VACV (105 pfu) (a) or ECTV (10 pfu of WT and 106 pfu of the IFNα/βBP mutants) (b), respectively. Mice were monitored daily for survival, bodyweight and signs of illness, and footpad swelling in the case of ECTV. Weight data are expressed as the mean ±SD of the five animals weights compared to their original weight at the day of inoculation. Signs of illness, as a score ranging from 1 to 4, and the size of the footpad (mm) where inoculation was performed are also expressed as the mean of the five animals. Statistical analyses performed were multiple t-tests with FDR Q = 1% and days at which significant differences (p < 0.05) were found between IFNα/βBPGAGmut and WT or ∆IFNα/βBP infections are indicated with solid or dotted lines, respectively. One representative experiment of two is shown