Fig. 8.

Detection and biological action of the IFNα/βBP in plasma from infected animals. Dilutions containing 20% of plasma from mice infected with the indicated viruses at 5 dpi were independently incubated with 50 U of human IFNαA for VACV samples or 50 U of mouse IFNαA for ECTV samples, and then added to HeLa or L929 cells, respectively. After 16 h cells were infected with VSV and cell viability measured at 72 hpi to determine the IFN blocking activity from plasmas. Mean±SEM from a representative experiment in triplicate from two with 5 mice/group is shown in the left panels, while the corresponding individual values obtained for each mouse are shown in the right panels