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. 2018 Dec 21;9:5426. doi: 10.1038/s41467-018-07126-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Phd2 deletion induced HIF target and glucose uptake changes. a Phd2 regulated gene expression in mouse melanomas. Quantitative RT-PCR assay for Phd2, CAIX, VEGFA, GLUT1, PGK, PGM and LDHA mRNA expression in Tyr::CreER; BRaf^V600E; Phd2^−/− mice melanoma tissues (n = 3 replicate experiments; *p < 0.01 compared with Tyr::CreER; BRaf^V600E; Phd2^−/+ or Tyr::CreER; BRaf^V600E; Pten^−/− mouse melanoma tissues). β-Actin is used as loading control. b Phd2 regulated protein expression in mouse melanomas. Expression of Phd2, HIF-1α, VEGFA, pAkt and Akt protein was determined by western blot analysis in Tyr::CreER; BRaf^V600E; Phd2^−/−, Tyr::CreER; BRaf^V600E; Phd2^-/+ and Tyr::CreER; BRaf^V600E; Pten^−/− mouse melanoma tissues (n = 3 replicate experiments). β-Actin was used as a loading control. c Uptake of a fluorescent deoxyglucose analog (2-NBDG) is increased in Phd2^−/− mouse melanoma cells. The 2-NBDG uptake assay was performed using BRaf^V600E; Phd2^−/− or BRaf^V600E; Pten^−/− mouse melanoma cells (left panel) (n = 3 replicate experiments). Quantity of 2-NBDG uptake is assessed in these mice (right panel) (n = 3 replicate experiments, *p < 0.05). d HIF inhibition reverses Phd2 deletion-induced glucose uptake increase. 2-NBDG uptake is assessed in FM19G11-treated BRaf^V600E; Phd2^−/− melanoma cells (n = 3 replicate experiments, *p < 0.05 compared with control group). e Knockdown of HIF-1α reverses Phd2 deletion-induced glucose uptake increase. 2-NBDG uptake is assessed in BRaf^V600E; Phd2^−/− melanoma with HIF-1α knockdown (n = 3 replicate experiments, *p < 0.05 compared with control group). One way ANOVA or t-test was used for statistical analysis and error bars indicate s.d.