Fig. 2.

Effect of WEPN on RANKL-induced osteoclast differentiation in BMMs. BMMs were cultured with vehicle (distilled water) or WEPN (5–80 μg/mL) in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/mL) for 4 days. (A) Cells were stained for TRAP staining. (B) BMMs were cultured with or without WEPN in the presence of M-CSF for 2 days, and cell viability was determined using Cell Counting Kit-8 assay. (C) TRAP activity of osteoclasts were analyzed as described in Section 2. (D) TRAP-positive multinucleated cells containing more than three nuclei and larger than 100 μm in diameter were counted as osteoclasts. ***p < 0.001 vs vehicle-treated control.