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Effect of WEPN on RANKL signaling pathway in BMMs. (A) BMMs were pretreated with WEPN (80 μg/mL) in the presence of M-CSF (60 ng/mL) for 1 h and then incubated with RANKL (100 ng/mL) for indicated time points. Total cell lysate (30 μg) was subjected to Western blot analysis with the indicated antibodies. (B) mRNA expression levels of c-Fos, NFATc1, TRAP, cathepsin K, DC-STAMP, and carbonic anhydrate were analyzed by qPCR with specific primers. ***p < 0.001.
