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. 2018 Dec 21;8:18024. doi: 10.1038/s41598-018-36626-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced triglyceride (TG) turnover is observed in TLR4^−/− TB-mice. (A) Primary adipocytes from scAT were incubated in the presence (stimulated) or absence (basal) of isoproterenol (10⁻⁵M) for 1 hour. Graph shows lipolysis response assessed by the ratio of glycerol released (nmol/10⁴ cells) relative to the basal condition. N = 4 per group. (B) Serum concentrations of non-esterified fatty acids (NEFA) and (C) % of palmitic acid in scAT were performed for each different group. N = 4 per group. (D) Immunoblot analysis for components of lipolysis. scAT lysates were immunoblotted for phospho-HSL (Ser660) and total HSL (E) Densitometric evaluation of phospho-HSL (Ser660) and total HSL. Ponceau staining was analyzed as a loading control. N = 4 per group. (F) qRT-PCR was performed to quantitate Pck1, Acsl1, Acads, Citrate Synthase, Me3 and Cpt1b mRNA levels in scAT from the different groups. N = 5 per group. Graph show the mean ± SEM. Statistical significance was determined by two-way ANOVA. *P < 0.01; ***P < 0.001.