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. 2018 Dec 21;8:18024. doi: 10.1038/s41598-018-36626-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TLR4 deletion reduces browning effect in TB mice throughout p38MAPK pathway. (A) Representative images of UCP1 staining of scAT from the different experimental groups. N = 5 per group. (B) Total quantification of UCP1 staining. (C) qRT-PCR was performed to quantitate Ucp1, Pgc1a, Prdm16, Cidea, Cidec, Adrb1 and Adrb3 mRNA levels in scAT from the different groups. N = 5 per group. (D) Depicted are representative immunoblots to detect phospho-p38MAPK, p38MAPK, phospho-AMPK and AMPK levels in scAT from the different experimental groups. N = 4 per group. (E) Densitometric evaluation of protein levels (phospho/total). Scale bars, 200 μm. Graphs show the mean ± SEM. Statistical significance was determined by two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.