Table 2.
LC-MS/MS Analysis of Disulfide Bridges in Human Alkbh5
| Sample | 6 M GuHCl | −IAM (Da)a | +IAM (Da)b | ΔMass (Da)c | Alkylated Cysd |
|---|---|---|---|---|---|
| Alkbh5 | No | 26,374 | 26,602 | 228 | 4 |
| Yes | 26,374 | 26,659 | 285 | 5 | |
| Alkbh5 + Mn2+ αKG | No | 26,374 | 26,602 | 228 | 4 |
| Yes | 26,374 | 26,659 | 285 | 5 | |
| Alkbh5 + Mn2+ αKG + GG(m6A)CT | No | 26,374 | 26,374 | 0 | 0 |
| Yes | 26,374 | 26,659 | 285 | 5 |
a
This is the mass of Alkbh5 before addition of the alkylating agent IAM.
b
This is the mass of Alkbh5 after addition of the alkylating agent IAM.
c
This is the mass shift caused by addition of IAM.
d
The number of alkylated cys was calculated by dividing ΔMass by 57 Da (the theoretical mass shift per alkylated Cys) (40).