Table 1.
Diffusional States of Kip3
Single Kip3, 1 mM ATP, υ = 62 nm/s | |||
---|---|---|---|
State of motion | Forward | Slow | Fast |
Diffusion constant D (deg2/s) | 4.2 ± 0.7 | 52 ± 4 | 490 ± 54 |
Relative abundance (%) | 18 ± 2 | 55 ± 3 | 27 ± 2 |
Weighted diffusion constant (deg2/s) | 162 ± 28 | ||
Single rkin430, 10 μM ATP, υ = 187 nm/s | |||
Diffusion constant (deg2/s) | 5.0 ± 0.5a |
The value was normalized to the speed of single Kip3 (Supporting Materials and Methods, Section 1).